hyclone HYCLONE HyClone 培养基 DMED 高糖 低糖 DMEM/F12 1640 抗体 PBS 胰酶 双抗 PS 血清 胎牛血清 新西兰胎牛血清 hamk's 酚红 缓冲液 PBS缓冲液 hamk‘s 低糖 高糖 RPMI EDTA 澳洲血清 澳胎 南美胎牛血清 南美 乌拉圭血清 DMEM HIGH 试剂盒 Human mouse rat WB IHC 耗
The Invitrogen Life Technologies TRIzol Reagent (Total RNA Isolation Reagent) is a ready-to-use reagent for the isolation of total RNA from cells and tissues for use in PCR analysis. TRIzol reagent is a mono-phasic solution of phenol and guanidine isothiocyanate. During tissue homogenization or lysis, the TRIzol
Cells were grown on 0.2% gelatinized (Sigma, G1890) tissue culture plates in 2i media, DMEM-F12 (Life Technologies, 11320082), 0.5X B27 supplement (Life Technologies, 17504044), 0.5X N2 supplement (Life Technologies, 17502048), an extra 0.5mM L-glutamine (Gibco, 25030-081), 0.1mM b-mercaptoethanol (Sigma, M7522), 1% Penicillin Streptomycin ...
(Gibco Formula) Equipment: 1 liter Beaker Stir plate & stir bar Sterile 500ml bottles (x2) 0.2 µm Vacuum Filter Reagents: 1000 ml Distilled Water 400 mg Potassium Chloride (KCl, FW=75; Sigma #P-4504) 60 mg Potassium Phosphate, monobasic (KH 2PO 4, FW=136; Sigma #P-8416) 350 mg Sodium Bicarbonate (NaHCO 3, FW=84; Sigma #S-5761) The technologies that support three-dimensional (3-D) suspension culture, such as a stirred bioreactor, are generally considered as promising approaches to produce the required cells. Recently, suspension bioreactors have also been used to generate mini-brain-like structure from hPSCs for disease modeling, showing the important role of ...